Can I set the ploidy of the data? Helen June 16, 2020 21:44 Yes, as of version 1.1 this option can be found in the Locus Info window. Related articles How do I change the start position or numbering of my sequence? Geneious Prime prompts me to remove a bad (250bp) peak? Where can I find the manual for the Microsatellites plugin? Getting started with microsatellites in Geneious Prime How can I find all my Locus Documents? Comments 1 comment Sort by Date Votes Abhi Rath December 25, 2020 04:25 Hi Helen, I have been using GENEIOUS for MSI calling for quite some time now (a year or so) and I still could not figure out how to make it recognize heterozygous alteration where the data still retains the WT copy but the other allele is altered....even if I adjust the expected peak to 2 because of diploid cells. It creates an extra column but does not show anything. Ideally, GENEIOUS should recognize the normal allele and the altered allele....but it does not. So, what it has come down to that we have to analyze each and every peak manually....just to make sure that there was a heterzoygous change for a certain marker but GENEIOUS did not pick it up and called the marker length to be WT. So, any help in this regard will be greatly appreciated. Thanks much, Abhi 0 Please sign in to leave a comment.