Molecular Cloning and Primer Design Gabriel Ortiz August 12, 2021 03:40 Updated One-step GoldenGate, homology-based (Gibson, GeneArt, In-Fusion etc), TOPO and restriction cloning. Restriction maps, codon optimization, silent mutation analysis, simulated PCR, digestion and ligation. Primer design and testing. CRISPR. Related articles How do I design cloning primers? Sequence Analysis How to add primers to Geneious Prime How do I import a custom codon usage table? Phylogenetic Tree Building Comments 0 comments Please sign in to leave a comment.