One-step GoldenGate, homology-based (Gibson, GeneArt, In-Fusion etc), TOPO and restriction cloning. Restriction maps, codon optimization, silent mutation analysis, simulated PCR, digestion and ligation. Primer design and testing. CRISPR.
One-step GoldenGate, homology-based (Gibson, GeneArt, In-Fusion etc), TOPO and restriction cloning. Restriction maps, codon optimization, silent mutation analysis, simulated PCR, digestion and ligation. Primer design and testing. CRISPR.