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Can Geneious Prime find nicking enzyme (endonuclease) sites?

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2 comments

  • Dirk Becker

    most entries from the neb file (withrefm.just_nicks) are not imported by Geneious. Apparently problems in recognizing the '?' in the cut/nick position identifier....

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  • Moreland Gibbs

    Dirk, thanks for raising this issue. I was not aware Geneious was excluding all sites with a ?
    I have revised the list to limit it to just the 15 commercially available nicking enzymes (according to http://rebase.neb.com/rebase/azlist.nick.cy.html), and removed the nick site position information for each enzyme. The revised list (attached) will allow identification and annotation of all 15 enzyme recognition sites, but the nick position will not be shown.

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