Remove sequences of certain length.

INDERPREET SINGH

• May 05, 2021 04:29

 I need to do the following steps
1. Set pair reads
2. Remove duplicate reads
3. Merge paired reads
4. Error correct and normalise reads
5 remove sequence in each data , which is lower than 151 nucleotides and more than 320 nucleotides.
I have the problem in adding the last step in the workwflow

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  Hilary Miller

  • May 05, 2021 23:09

  You can extract sequences of the length you want using the Lengths Graph above the viewer, as shown in the screenshot below:

  

  If you want to automate this in a workflow, you need to do two filtering steps - one to remove the short reads and the other to remove the longer reads.  

  

  The workflow setup would look something like in the screenshot below:
  

  This produces a sequence list with the same names as the input document but containing only sequences between 151 and 320 bp.  

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