After pairwise alignment (MAFFT, MUSCLE is not working) I transferred the annotation to other sequence. But whenever I tried to import this sequence in gene bank file then all the mutations and gap stripped. Thus, the base pair also reduced and lost sequence overall integrity. But if I keep this file in geneious format then its alright. So my query is how can I make GB file keeping all things as it is.
Thanks a lot for your assistance.
Please sign in to leave a comment.