How can we help?
Announcements
- Is Geneious Prime compatible with MacOS Monterey?
- New online license management with My Account
- InterProScan plugin not currently working
- Issues with downloading BLAST search results from PDB - December 2020
- Windows Users: Geneious Prime 2020 and above will not support 32-bit Windows
- Uniprot search returns no results
Knowledge Base
Installing Geneious Prime
- Supported Operating Systems
- Minimum system requirements for running Geneious Prime
- Is Geneious Prime compatible with macOS 11.0 Big Sur?
- Is Geneious Prime compatible with Apple M1 chips?
- Does Geneious Prime run on Ubuntu 18.04 LTS?
- Mac Users: Running Geneious/Geneious Prime on macOS 10.15 Catalina and higher
General
- Where can I find the manual for Geneious Prime?
- How do I cite Geneious or Geneious Prime in a paper?
- Where can I find tutorials for Geneious Prime?
- Where are the release notes for Geneious?
- Has Geneious Prime been validated? Does it comply with the FDA regulation 21 CFR Part 11?
- Does Oracle's recent changes to the Java licensing model affect commercial usage of Geneious solutions?
Geneious databases
- Where is my Geneious Prime database stored?
- How do I change where my Geneious Prime database is stored?
- How do I back up the local database?
- How do I restore a Geneious backup?
- How can I access my database from different computers?
- How can multiple users access the same database?
Licensing
- How does a Geneious Prime subscription plan work?
- How do I renew my subscription plan?
- What happens when my subscription expires?
- Whats the difference between a Personal license, a Group license, and a Floating License?
- What’s the best licensing package for my lab?
- Am I eligible for student pricing?
Geneious Floating License Manager
- What is the Geneious Floating License Manager?
- How do I upgrade to a new version of the Floating License Manager?
- Geneious Floating License Manager download links and installation instructions
- How do I move my Floating License manager (FLM)?
- How do I activate a new FLEXnet Floating License on the Geneious Floating License Manager?
- I can’t connect to the Floating license manager (License server)
Geneious Performance and Memory Issues
- Geneious Prime is slow or frequently runs out of memory
- How do I allocate more RAM to Geneious Prime?
- How can I see how much memory Geneious Prime is using?
- Why can’t I allocate more than 1 GB of RAM to Geneious Prime?
- Why does Geneious Prime appear to be using lots of memory when I’m not running any analyses?
- Why does Geneious Prime appear to not be using any memory when I’m running a large phylogenetic analysis?
Geneious Prime functionality - General
- How to run Geneious operations via the command line
- Can I run Geneious in dark mode?
- How to search and filter in Geneious Prime
- Can Geneious Prime handle RNA-Seq data?
- How do I script or automate Geneious Prime?
- Will Geneious Prime run multi-threaded? How do I make my analysis run across multiple cores?
Viewing and Editing Sequences
- Using Annotate by BLAST to annotate nucleotide sequences
- Shortcut keys for sequence navigation and editing
- How do I display amino acid numbering on a nucleotide sequence?
- Annotating your sequence from a custom annotation database.
- How do I circularize my linear sequence?
- How do I change the start position or numbering of my sequence?
Importing and Exporting Data
- How do I get my Vector NTI data into Geneious Prime?
- How do I import metadata from a spreadsheet onto existing Geneious documents?
- How do I export my sequence or alignment as formatted text? [Updated for Prime 2019.2]
- How do I export publication-quality images?
- Geneious vs GenBank: Nomenclature and exporting of Geneious Prime files in GenBank format
- What data file types can be imported?
Alignment and Assembly
- Best practice for preprocessing NGS reads in Geneious Prime
- How do I assemble F and R pairs of Sanger sequences?
- Assembly of SARS-CoV-2 genomes from tiled amplicon Illumina sequencing using Geneious Prime
- What are the hardware requirements for assembly of NGS data using the Geneious de novo Assembler?
- Can Geneious Prime assemble PacBio or Minion data?
- Can I perform a hybrid assembly with Illumina and PacBio/MinION data?
Phylogenetics
NCBI and BLAST
- How to use an NCBI API key for NCBI Search & Blast
- How can I BLAST against my own sequences or a database that isn’t part of NCBI?
- How can I BLAST to a local copy of preformatted NCBI databases?
- I can't connect to NCBI BLAST and/or download from NCBI databases
- Error Search failed - NCBI received too many requests from your IP
- How to restrict a BLAST search to a specific organism when searching NCBI
Primers
- How to add primers to Geneious Prime
- A quick guide to importing primer sets from TSV/CSV files
- How do I design degenerate primers based on an alignment?
- Designing Taqman® and qPCR primers in Geneious Prime
- How do I design cloning primers?
- Does Geneious Prime support inosine?
Microsatellites
- Getting started with microsatellites in Geneious Prime
- Geneious Prime doesn’t recognise my ladder - how do I fix it?
- Can I use a custom microsatellite ladder?
- What dyes does Geneious Prime recognise?
- Can I set the ploidy of the data?
- Geneious Prime prompts me to remove a bad (250bp) peak?
Cloning
- How do I set the off-target database for CRISPR?
- How do I create a custom enzyme set?
- How do I import a custom codon usage table?
- How do I find non-cutting restriction enzymes?
- How do I annotate promoters, antibiotic resistance sites and other features onto my plasmid?
- Why can’t Geneious Prime find my restriction enzyme lists?
Geneious Error Messages
- What should I do if Geneious Prime crashes or behaves strangely?
- License Activation error: Activation library initialization failed
- Downloaded command line tools fail on macOS with "can't be opened because its integrity cannot be verified"
- When I search online databases I get an error saying “Please check your connection settings”
- Operations Table errors
- “Failed to load document contents” errors
Linux
Plugin Development
Features at a Glance
- NGS Pre-Processing and Analysis
- Data Management and Collaboration
- Mapping and De Novo Assembly
- Sequence Alignment
- Searching and BLAST
- Sequence Analysis
Purchasing
- What is the price of Geneious Prime?
- Will I get an invoice?
- Can I purchase a student license under my name (PI) for my student?
- Will my credit card be charged again after the subscription ends?
- How do I know if I have I filled out the purchasing form correctly?
- How is the license key sent?