Custom ladders can be added from plugin version 1.2 onwards. Ladders are stored in a text ﬁle which can be manually edited to add new ladders.
The ladders.txt ﬁle contains a tab-separated list of ladders where the ﬁrst ﬁeld is the name of the ladder and the rest are the ladder fragment peak sizes in bp. If you edit this file then Geneious must be restarted for changes to take effect.
Locating and editing the ladders.txt
First, ensure you have installed the Microsatellites plugin is installed via menu Tools -> Plugins.
The ladders.txt ﬁle can be found in the microsatellite plugin installation directory:
If you do not have admin privileges it will be installed in your home directory in
Geneious X.Y data/Plugins
Within this directory you will find the microsatellites plugin folder named:
Within this folder you will find a series of subfolders:
and within data directory you will find the ladders.txt ﬁle.
If the plugin folder cannot be found in your data folder, try the following locations:
On a mac:
Note: The Appdata folder is a hidden folder. To find it you will have to go to Folder Options in the Windows Control Panel, and in the View panel and check the option to "show hidden files, folders and drives".