Installing Geneious Prime
- Supported Operating Systems
- Minimum system requirements for running Geneious Prime
- How do I update to the latest version of Geneious Prime?
- How do I download the latest update to Geneious Prime?
- Where can I find installers for older versions of Geneious?
- Downgrading to an earlier version of Geneious
General
- Where can I find the manual for Geneious Prime?
- How do I cite Geneious or Geneious Prime in a paper?
- Where can I find tutorials for Geneious Prime?
- Where are the release notes for Geneious?
- Has Geneious Prime been validated? Does it comply with the FDA regulation 21 CFR Part 11?
- Does Oracle's recent changes to the Java licensing model affect commercial usage of Geneious solutions?
Geneious databases
- Geneious Prime detected "verify/._verify.dat" in your Geneious data location
- Where is my Geneious Prime database stored?
- How do I change where my Geneious Prime database is stored?
- How do I back up the local database?
- How do I restore a Geneious backup?
- How can I access my database from different computers?
Licensing
- How to activate a Geneious Prime license (Where is my License Key)?
- Activation Issues: Unable to reach the license server (Email Sign-on)
- My license has already been activated the maximum number of times - what can I do?
- What are the differences between available license types?
- How do I release a license from Geneious?
- Can I activate my personal Geneious license on more than one computer?
Geneious Floating License Manager
Shared Databases
- Recommended SQL configurations for Shared Databases
- How to set up a Shared Database using PostgreSQL
- How to set up a Shared Database using Amazon (AWS) RDS
- How do I backup my Shared Database or Geneious Server Database?
- Can I use Windows Authentication to log into my Shared Database?
- How to share links to documents in the Shared Database
Geneious Cloud
Geneious Performance and Memory Issues
- Geneious Prime is slow or frequently runs out of memory
- How do I allocate more RAM to Geneious Prime?
- How can I see how much memory Geneious Prime is using?
- Why can’t I allocate more than 1 GB of RAM to Geneious Prime?
- Why does Geneious Prime appear to be using lots of memory when I’m not running any analyses?
- Why does Geneious Prime appear to not be using any memory when I’m running a large phylogenetic analysis?
Geneious Prime functionality - General
- How to run Geneious operations via the command line
- Can I run Geneious in dark mode?
- How to search and filter in Geneious Prime
- Can Geneious Prime handle RNA-Seq data?
- How do I script or automate Geneious Prime?
- Will Geneious Prime run multi-threaded? How do I make my analysis run across multiple cores?
Viewing and Editing Sequences
- Using Annotate by BLAST to annotate nucleotide sequences
- Shortcut keys for sequence navigation and editing
- How do I display amino acid numbering on a nucleotide sequence?
- Annotating your sequence from a custom annotation database.
- How do I circularize my linear sequence?
- How do I change the start position or numbering of my sequence?
Importing and Exporting Data
- I can't import genbank (.gbk) files from Prokka
- Search sequences in Dotmatics Bioregister
- Register sequences in Dotmatics Bioregister
- How to batch export individual plasmid maps as PDF/image files.
- How do I get my Vector NTI data into Geneious Prime?
- How do I import metadata from a spreadsheet onto existing Geneious documents?
Alignment and Assembly
- Muscle 5.1 failed with error code -1073741515
- Geneious Map-to-Reference assembly advisor decision tree & validation table
- Geneious de novo assembly advisor decision tree & validation table
- Best practice for preprocessing NGS reads in Geneious Prime
- How do I assemble F and R pairs of Sanger sequences?
- Assembly of SARS-CoV-2 genomes from tiled amplicon Illumina sequencing using Geneious Prime
Phylogenetics
NCBI and BLAST
- How to add the NCBI Blast Core Nt Database
- I can't access NCBI Genome database (October 2024)
- How can I BLAST against my own sequences or a database that isn’t part of NCBI?
- How can I BLAST to a local copy of preformatted NCBI databases?
- I can't connect to NCBI BLAST and/or download from NCBI databases
- How to restrict a BLAST search to a specific organism when searching NCBI
Primers
- How to add additional NCBI databases for performing custom BLAST searches
- How to perform specificity testing during Primer Design
- How to add primers to Geneious Prime
- A quick guide to importing primer sets from TSV/CSV files
- How do I design degenerate primers based on an alignment?
- Designing Taqman® and qPCR primers in Geneious Prime
Microsatellites
- Getting started with microsatellites in Geneious Prime
- Geneious Prime doesn’t recognise my ladder - how do I fix it?
- Can I use a custom microsatellite ladder?
- What dyes does Geneious Prime recognise?
- Can I set the ploidy of the data?
- Geneious Prime prompts me to remove a bad (250bp) peak?
Cloning
- How to use Gibson Assembly to simulate swapping parts between vectors.
- How do I delete or remove custom restriction enzyme lists?
- How to use the Cloning Validation tool
- How do I set the off-target database for CRISPR?
- How do I create a custom enzyme set?
- How do I import a custom codon usage table?
Geneious Error Messages
- M-series Mac: error=86, Bad CPU type in executable
- What should I do if Geneious Prime crashes or behaves strangely?
- Downloaded command line tools fail on macOS with "can't be opened because its integrity cannot be verified"
- When I search online databases I get an error saying “Please check your connection settings”
- Operations Table errors
- “Failed to load document contents” errors
Linux
- Which Linux configurations are supported?
- How can I setup Geneious Prime to analyse RNA-Seq using DESeq2, on Linux?
- How do I run the Phobos plugin in Linux?
- Some functionality in Geneious R10 and earlier (e.g. Primer design, RAxML, EMBOSS tools) doesn't work under 64-bit Linux.
- The web links inside Geneious Prime don’t work under Linux
Plugin Development
Purchasing
- Entity Change and Banking Information for Geneious
- What is the price of Geneious Prime?
- Will I get an invoice?
- Can I purchase a student license under my name (PI) for my student?
- Will my credit card be charged again after the subscription ends?
- How do I know if I have I filled out the purchasing form correctly?