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  3. Cloning

Cloning

  • How to use Gibson Assembly to simulate swapping parts between vectors.
  • How do I delete or remove custom restriction enzyme lists?
  • How to use the Cloning Validation tool
  • How do I set the off-target database for CRISPR?
  • How do I create a custom enzyme set?
  • How do I import a custom codon usage table?
  • How do I find non-cutting restriction enzymes?
  • How do I annotate promoters, antibiotic resistance sites and other features onto my plasmid?
  • Why can’t Geneious Prime find my restriction enzyme lists?
  • Can I use a custom virtual gel ladder?
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