We are currently aware of an issue with MacOS Big Sur where Geneious may crash after you restart your computer and will not reopen. Please see this article for more information about this issue.
There is a known issue when running Geneious Prime on MacOS Big Sur, where you may experience hangs when dialog windows are opened in a tabbed view. This is caused by a new setting in Big Sur in System Preferences → General called “Prefer tabs [never, when full-screen, always] when opening documents”. Setting this on "Never" should resolve this issue.
A second issue is related to the Find CRISPR Sites operation, where the Doench et al 2016 algorithm introduced in Prime 2021 to calculate Activity Scores may not run on Big Sur. Users on Intel macs may be able to fix this by upgrading their system install of pip for python 3 using the following command (run without sudo/admin privileges):
python3 -m pip install --upgrade pip
Older versions of Geneious (Prime 2020.2.4 and earlier) may experience stability issues on Big Sur if the computer is allowed to sleep or Geneious is idle for an extended period of time. This issue is fixed in Prime 2020.2.5 and Prime 2021 onwards.
Users on versions R9-R11 may also see an error associated with FLEXnet licensing upon opening Geneious on Big Sur for the first time, and that they are unable to activate their license. These licensing issues have been fixed in Geneious Prime 2019.2.3 and later, but users on R9-R11 perpetual licenses can install the licensing fix located here to fix the licensing component of Geneious.