Alignment and Assembly
- Geneious de novo assembly advisor decision tree & validation table
- Best practice for preprocessing NGS reads in Geneious Prime
- How do I assemble F and R pairs of Sanger sequences?
- Assembly of SARS-CoV-2 genomes from tiled amplicon Illumina sequencing using Geneious Prime
- What are the hardware requirements for assembly of NGS data using the Geneious de novo Assembler?
- Can Geneious Prime assemble PacBio or Minion data?
- Can I perform a hybrid assembly with Illumina and PacBio/MinION data?
- How do I set up Windows 10/11 to run SPAdes, Flye, or STAR assemblers?
- Which multiple alignment algorithm should I use?
- How do I delete all gaps in my alignment?
- Can I call SNPs on individual sequences aligned to a reference?
- How do I map peptides to a protein sequence?
- What’s the difference between Pairwise/Multiple alignment, de novo Assembly, and Map to Reference?
- Which de novo assembly algorithm is best for my data?
- Which map to reference assembly algorithm is best for my data?
- How do I strip alignment columns in R10 and above?
- Where can I get reference databases for chimera filtering?
- How do I assemble paired reads?
- Can the Geneious assembler do scaffolding?
- How do I use the advanced assembly settings?
- Can I use multiple reference sequences in my assembly?
- What’s the difference between soft trimming and hard trimming in Geneious Prime?
- Why is my alignment taking so long?
- Error: Your operating system won't allow Geneious to open enough files simultaneously to perform the operation